Enzymes Day 2 (requested content/links)

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http://www.carnegieinstitution.org/first_light_case/horn/MSI/
MSIenzymes/enzymesmsi.html

Link to DCPS unit planner template (Word Document)

• Create-a-Graph
• Graphing on Excel (MS 97 instructions), Techtorial, How to plot in Excel, and here
• Bio-math http://www.math.iupui.edu/mtc/Biology/Bio2.html

Protein catalysts are called enzymes. Proteins have structural features that give clues about their action.

• Enzymes are biological catalysts. http://chemeng.uah.edu/courses/che440sp97/enzyme.html
• Simple explanation, but OK http://www.historyoftheuniverse.com/enzywork.html
• Popular review (based on teacher responses) http://www.biology.arizona.edu/biochemistry/problem_sets/energy_enzymes_catalysis/energy_enzymes_catalysis.html
• Good college level description of enzyme action http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Enzymes.html another college course topic site http://eve.kean.edu/~breid/enzyme/enzyme.html
• Good upper level college review of enzymes Rick Hallick's site pages http://www.blc.arizona.edu/courses/181gh/rick/energy/enzymes1.html
• http://www.blc.arizona.edu/courses/181gh/rick/energy/enzymes2.html
• Catalysts speed up reactions without becoming products of the reaction.
• http://www.vaneyk.net/vaneyk.net/html/what_is_an_enzyme.html

Catalysts bind substrates (the molecules they act on) in a way that the particular bond(s) of each substrate molecule essentially stretch, reducing the activation energy required to form the reaction products.

How enzymes work

• Good diagram of biosynthetic enzyme action http://www.wheatgrass.com/book/chapter6.html
• Good animation of a polymer degrading enzyme (lysozyme or amylase) http://homepages.enterprise.net/caistorg/c_lyso.html
• http://www.freelance-animation.com/animations/enzyme_mov.html Silly but a cute intro
• http://web.ukonline.co.uk/webwise/spinneret/other/anenz.htm action of degrading enzyme on substrate
• http://web.ukonline.co.uk/webwise/spinneret/other/andnat.htm internal motion in the molecule that enables its action
• Action of a synthetic enzyme http://student.ccbc.cc.md.us/biotutorials/proteins/enzsub.html

Good graph of different catalytic reactions (substrates, products/intermediates) http://www.wheatgrass.com/book/chapter6.html

• http://www-biol.paisley.ac.uk/kinetics/Chapter_4/chapter4_2_3.html more complicated enzyme reaction (but like the atom exchange between NaOH and HCl)
• http://www.nmsu.edu/~molbio/diabetes/n-enzyme.html Enzyme removing a piece of a molecule converting it into another molecule. Enzyme (lack of) action on a mutant protein substrate (confusing imagery)
• http://www.nmsu.edu/~molbio/diabetes/m-enzyme.html

Change enzyme, substrate and equilibrium constants to see substrate go away and product appear in a time course experiment.http://www.rpi.edu/dept/chem-eng/Biotech-Environ/Canada/enzkin.html

Sequences and structures

human amylase, yeast catalase, potato catalase

• http://www.reachoutmichigan.org/funexperiments/quick/middlesch.html
• http://www.reachoutmichigan.org/funexperiments/quick/access/enzymereact.html
• http://www.reachoutmichigan.org/funexperiments/quick/eric/enzymes.html
• http://www.reachoutmichigan.org/funexperiments/quick/access/enzymegrab.html
• http://www.reachoutmichigan.org/funexperiments/quick/access/enzymejello.html
• http://www.reachoutmichigan.org/funexperiments/quick/access/enzymesub.html
• http://www.reachoutmichigan.org/funexperiments/quick/alcom/hotpacks.html
• cheese-making
• http://www.uoregon.edu/~smrandy/workshop/h2o2harris.html
• insulin 1ZEH, lysozyme 1HHL (egg white)

Other ways of testing for the enzymes: amylase, catalase, pepsin

potato and peroxidase