experiment with enzymes references

Science and Mathematics Workshop
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http://www.caseciw.org/first_light_case/horn/EWE/ewerefs.html

References for Experiment with Enzymes (includes studies of yeast)

The starting point! Roy B. Clariana (September 1991) pH & rate of enzymatic reactions. American Biology Teacher pp. 349-350 (not available online).
updated June 22, 2002

GRAPH PAPER web site! Thanks to Holly Camp at Athens State University. Math of Enzyme Action

General information on enzymes

Enzyme Primer from the Enzyme Technical Association (34 page readable pdf file)
Characteristics of enzymes
Enzyme action
Kinetics chapter (what happens a short time after the reaction starts)
Great links about enzymes (DNA in Action)
Another cool enzymes site (Detergent Action)
Enzyme techonology (how researchers study enzymes for industrial use)
How enzymes are named (enzyme nomenclature)
Enzyme biochemistry (from the first Internet science course)
Enzyme manual (biochemists would have the Worthington Manual as their reference for descriptions of all kinds of enzymes and how to assay for them!)
Government listing of enzyme reactions from the National Institutes of Standards and Technology
Comparing proteins based on their amino acid composition

Kathy Schrock's Guide for Educators (Internet sites, rubrics, assessment, etc.)
Careers of African American and Hispanic Enzyme researchers
Applications of enzymes in the real world here

Catalase in particular

Disc flotation test for monitoring catalase activity in milk
Robots Eavesdrop on Cellular Discussions (February 2000) Scientists use yeast as a model system for developing new technology.
On May 28, 2001, the National Center for Biotechnology Information listed 6345 references to professional research papers and reviews on catalase assay. There were 569 references on yeast catalase assay in the database.
Zebra fish catalase is related to yeast catalase and shows activity when the cDNA is transfected into bacteria
Effect of copper on catalase activity
Purification of yeast catalase (1973) (no abstract is available. open the menu where you see abstract; scroll to citation, then click on display)
Detection of catalase in polyacrylamide gels (1972, Bruce and Morris) (1974, Gregory and Fridovich)
Dr. Wilkins and Dr. Gregory of Virginia Tech studied catalases of Bacteroides (anaerobes) and here
PATENTS involving catalase
Catalysis and activation energy of a chemical reaction
Catalase, the perfect enzyme?

Modeling enzyme action

Toothpickase
Protein structure (word document)
Animation of how substrates become products (synthesis), here is an animation of hydrolysis (degradation)
(En francais) model and molecule animations
Diffusion
Modeling atoms into molecules (atomic cookies)

Catalases in:

Spinach
Beef
Sweet potato catalase mRNA
Maize response to stress and wounding
role of iron on catalases in biofilm microbes
Liver

Medical features of catalase: acatalasemia

Teacher-developed labs:

Catalase (Genevieve Nelson, Access Excellence)
Toothpickase (Peggy Skinner, Access Excellence)
Experiment with Enzymes: Catalase (Leslie Pierce (AE) Virginia Department of Education)(PDF file adobe acrobat needed)
Effect of temperature on yeast catalase
Catalase web site designed by pre-service teachers (University of Alabama, Huntsville - near Marshall Space Flight Center!)

Enzyme Kinetics: Kinetics describes reaction progress over time in the short term (seconds to hours)

Catalase kinetics (high school level) http://www.science-projects.com/catalasekinetics.htm
Setting up data tables http://utweb.utampa.edu/faculty/hborgeas/204Assignments/204LFigures&Tables.htm
A 26 page pdf file and fairly complete summary of enzyme kinetics/analysis http://www.chem.wayne.edu/~chm5600/lectures/Notes1-20300.PDF
Some possible people to contact http://biospace.intota.com/multisearch.asp?strSearchType=all&strQuery=enzyme%20analysis
A good introduction http://www.worthington-biochem.com/introBiochem/
The first cybertext http://esg-www.mit.edu:8001/esgbio/eb/ebdir.html
A self-test from the MIT hypertext http://www.md.huji.ac.il/MedChem/Mit-Course/sq.html
Problem sets from the MIT hypertext (challenging) http://esg-www.mit.edu:8001/esgbio/eb/problems.html http://www.biology.arizona.edu/biochemistry/problem_sets/energy_enzymes_catalysis/energy_enzymes_catalysis.html
A great simulation http://www.chem.uci.edu/education/undergrad_pgm/applets/sim/simulation.htm
Another simulation that has simple software to download or that can be run from the browser on a school's computer http://www.chembio.uoguelph.ca/educmat/chm258/chm258.htm
A slide show http://ull.chemistry.uakron.edu/genobc/Chapter_20/
A more complicated explanation but does show the basic graphs, etc http://www.umdnj.edu/biochweb/education/bioweb/EnzymeMichaelisMenten.htm
Great tutorial http://jeffline.tju.edu/CWIS/DEPT/biochemistry/kinetics/HTML/INDEX.HTML
Another tutorial (referenced in the lab manual) http://www-biol.paisley.ac.uk/kinetics/contents.html
Measuring Km and Vmax http://esg-www.mit.edu:8001/esgbio/eb/kinetics/MandM4.html
Historical algebra! http://dept.physics.upenn.edu/courses/gladney/mathphys/subsection4_1_6.html
http://ntri.tamuk.edu/cell/kinetics.html as close to the Cleland graphical analysis book as I have found
Statistical analysis packages http://www.spssscience.com/EK/index.cfm
Generating circularly permuted protein sequence and testing for enzyme activity!

About yeast

Yeast Proteins Get On the Map http://www.academicpress.com/inscight/03152002/grapha.htm

For the teacher (and students who are interested in teaching)

Working scientifically 2000
Inquiry and the National Science Education Standards 2000
Inquiring into Inquiry: Inquiry Learning and Teaching in Science, 2000
The Role of Scientists in the Professional Development of Science Teachers1996
Advisor, teacher, role model, friend: On being a mentor to students in science and engineering 1997
Singer M and Tuomi J1999 Selecting instructional materials
J. Myron Atkin, Paul Black, Janet Coffey, Classroom assessment and the national science education standards 2001
Virginia Tech's Institute for Connecting Science Research to the Classroom (coming --- Enzymes: Catalysts for Change)

For Fun AND Learning!

Dr. Art's BioSong Enzymes (to the tune of Blue Moon) http://www.biosongs.com/7.htm

Questions? Comments? Suggestions?

This page developed and maintained by Toby M. Horn, Ph.D., DC ACTS