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Experiment with Enzymes

A Practical Guide for Implementing the Secondary Science Standard
Investigate and Understand (SOL Bio.1 in Virginia)

Based on this original Article from The American Biology Teacher, September 1991(page 1, page 2)

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PREFACE
ACKNOWLEDGMENTS
Keywords for Outcomes of Ability in Scientific Process Skills
Teaching Methods Keywords
Constructivism and the Five E¹s

 

INTRODUCTION Part 1

 

INTRODUCTION Part 2

Enzymes in Our Daily Lives
Enzyme Analysis in the Professional Research Laboratory
Flow of an Enzyme Characterization (Activity Profile) Experimental Pathway
Scientific Tinkering
Yeast and Genetic Engineering
ONE: OBSERVATIONS OF LIVING THINGS ARE RECORDED IN THE LAB AND IN THE FIELD ...
Materials for Each Lab Station
Working with the Yeast Catalase Assay Materials
Teamwork in the Laboratory
TWO: HYPOTHESES ARE FORMULATED BASED ON OBSERVATIONS ...
THREE: VARIABLES ARE DEFINED AND INVESTIGATIONS ARE DESIGNED TO TEST HYPOTHESESŠ
Considerations in Experimental Design
When (Chemical) Solutions are the Problem
Concentration vs. Amount
Estimates and Ranges
FOUR: CARRY OUT THE CLASS EXPERIMENT ...
FIVE: GRAPHING AND ARITHMETIC CALCULATIONS ARE TOOLS FOR DATA ANALYSISŠ
Data Analysis Terms
Mechanics and Conventions for Graphing Data
Graphical Analysis of Enzyme Data
SIX: CONCLUSIONS ARE FORMED BASED ON RECORDED DATA, IMPACTS OF EXPERIMENTAL ERROR INHERENT IN EXPERIMENTAL DESIGN ARE IDENTIFIED AND DISCUSSED, AND VALIDITY OF DATA IS DETERMINEDŠ
Sample Data from a Class Experiment
SEVEN: ALTERNATIVE MODELS ARE RECOGNIZED AND ANALYZEDŠ

 

An Enzyme Purification Pathway
EIGHT: STUDENTS RESEARCH POPULAR AND SCIENTIFIC LITERATURE ...
Reading a Scientific Paper
Internet Research
NINE: APPROPRIATE TECHNOLOGY IS USED FOR GATHERING AND ANALYZING DATA AS STUDENT TEAMS DESIGN AND FOLLOW THEIR OWN EXPERIMENTAL PATHWAYSŠ
TEN: STUDENTS WILL USE APPROPRIATE TECHNOLOGY FOR COMMUNICATING RESULTS ...
SOURCES FOR REAGENTS AND EQUIPMENT Materials List for Experiments
APPENDIX
 
Teacher Notes
Template for 24-Well Dish
Biology 1 Processes Unit: Investigating Yeast Catalase
Science Laboratory Rubric
Student Worksheet
Student Self Assessment of Independent Investigations with Catalase
International Baccalaureate Experimental Sciences Assessment Criteria
Additional Internet Resources