Experiment with Enzymes

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A Practical Guide for Implementing the Virginia SOL Bio.1
Investigate and Understand

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Developed by Toby Horn and Leslie Pierce under contract to the Virginia Department of Education, 1999-2000

 
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See how one rural Virginia school adapted Experiment with Enzymes in Math and Science

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PREFACE
ACKNOWLEDGMENTS

Keywords for Outcomes of Ability in Scientific Process Skills

Teaching Methods Keywords

Constructivism and the Five E's

INTRODUCTION
(Includes scheduling advice)

Your Introduction for Students
Enzymes in Our Daily Lives
Enzyme Analysis in the Professional Research Laboratory
Flow of an Enzyme Characterization (Activity Profile) Experimental Pathway
Scientific Tinkering
Yeast and Genetic Engineering
ONE: OBSERVATIONS OF LIVING THINGS ARE RECORDED IN THE LAB AND IN THE FIELD ...
Materials for Each Lab Station
Working with the Yeast Catalase Assay Materials
Teamwork in the Laboratory
TWO: HYPOTHESES ARE FORMULATED BASED ON OBSERVATIONS ...
   
THREE: VARIABLES ARE DEFINED AND INVESTIGATIONS ARE DESIGNED TO TEST HYPOTHESES...
Considerations in Experimental Design
When (Chemical) Solutions are the Problem
Concentration vs. Amount
FOUR: CARRY OUT THE CLASS EXPERIMENT ...
Estimates and Ranges
FIVE: GRAPHING AND ARITHMETIC CALCULATIONS ARE TOOLS FOR DATA ANALYSIS...
Data Analysis Terms
Mechanics and Conventions for Graphing Data
Graphical Analysis of Enzyme Data
SIX: CONCLUSIONS ARE FORMED BASED ON RECORDED DATA, IMPACTS OF EXPERIMENTAL ERROR INHERENT IN EXPERIMENTAL DESIGN ...
Sample Data from a Class Experiment
SEVEN: ALTERNATIVE MODELS ARE RECOGNIZED AND ANALYZED
An Enzyme Purification Pathway
EIGHT: STUDENTS RESEARCH POPULAR AND SCIENTIFIC LITERATURE ...
Reading a Scientific Paper
NINE: APPROPRIATE TECHNOLOGY IS USED FOR GATHERING AND ANALYZING DATA AS STUDENT TEAMS DESIGN AND FOLLOW THEIR OWN EXPERIMENTAL PATHWAYS...
Internet Research
TEN: STUDENTS WILL USE APPROPRIATE TECHNOLOGY FOR COMMUNICATING RESULTS...
   
APPENDIX
 
SOURCES FOR REAGENTS AND EQUIPMENT
Materials List for Experiments
Teacher Notes

Template for 24-Well Dish

(Word document that you can modify)

Biology 1 Processes Unit: Investigating Yeast Catalase
Science Laboratory Rubric
Student Worksheet
Student Self Assessment of Independent Investigations with Catalase
International Baccalaureate Experimental Sciences Assessment Criteria
Additional Internet Resources

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